Review

atcc 10832 strain (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC atcc 10832 strain
Atcc 10832 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staphylococcus+aureus+strains+10832/Staphylococcus+aureus+subsp%2E+aureus+Rosenbach/10__7554_slash_elife__67301-237-19-19
Average 95 stars, based on 167 article reviews

atcc 10832 strain - by Bioz Stars, 2026-09

95/100 stars

Images

Cell Culture:

Article Title: Highly Stable Aptamers Selected from a 2′-Fully Modified fGmH RNA Library for Targeting Biomaterials
Article Snippet: .. Staphylococcus aureus strains 10832 (subsp. aureus Rosenbach, Wood 46, NRA105) and 12598 (subsp. aureus Rosenbach, NCTC 8530, Cowan I, NRS104) were obtained from ATCC (Manassas, VA) and cultured in Luria-Bertani (LB) broth for all experiments. .. Citrate BioPure ™ silver nanoparticles (10 nm diameter) were purchased from NanoComposix (San Diego, CA).

Image Search Results

( A ) Human IgG1 antibodies are large (150 kDa) proteins, consisting of two functional domains. The fragment antigen binding (Fab) region confers antigen specificity, while the crystallizable fragment (Fc) region drives interactions with the immune system. Each IgG1 is composed of two identical heavy chains and two identical light chains, which all consist of a constant (CH, CL) and a variable (VH, VL) domain. A panel of six human IgG1 mAbs that recognize polysaccharide and protein components on the cell surface of S. aureus and two nonspecific isotype controls was produced. Variable heavy (VH) and light (VL) chain sequences obtained from different scientific and patent publications were cloned in homemade expression vectors containing human heavy chain (HC) and light chain (LC) constant regions, respectively. ( B, C ) Biofilms of Wood46 ( B ) and LAC∆ spa ∆ sbi ( C ) were grown for 24 hr and incubated with 66 nM F598-IgG1 or ctrl-IgG1 (G2a-2). mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments. A ratio paired t-test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Human IgG1 antibodies are large (150 kDa) proteins, consisting of two functional domains. The fragment antigen binding (Fab) region confers antigen specificity, while the crystallizable fragment (Fc) region drives interactions with the immune system. Each IgG1 is composed of two identical heavy chains and two identical light chains, which all consist of a constant (CH, CL) and a variable (VH, VL) domain. A panel of six human IgG1 mAbs that recognize polysaccharide and protein components on the cell surface of S. aureus and two nonspecific isotype controls was produced. Variable heavy (VH) and light (VL) chain sequences obtained from different scientific and patent publications were cloned in homemade expression vectors containing human heavy chain (HC) and light chain (LC) constant regions, respectively. ( B, C ) Biofilms of Wood46 ( B ) and LAC∆ spa ∆ sbi ( C ) were grown for 24 hr and incubated with 66 nM F598-IgG1 or ctrl-IgG1 (G2a-2). mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments. A ratio paired t-test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Functional Assay, Binding Assay, Produced, Clone Assay, Expressing, Incubation, Labeling, Fluorescence, Control

( A, C ) Biofilm of S. aureus strain LAC ∆spa∆sbi ( A ) and Wood46 ( C ) was grown for 24 hr with buffer or DNase (1 mg/mL). DspB (30 nM) was added after 24 hr of biofilm formation. Adherent biofilm biomass was measured by crystal violet staining. Data represent mean + SD of three independent experiments. Triplicates were averaged and expressed as relative biomass by dividing the OD595 of treated samples by the OD595 of control samples. One-way ANOVA followed by Dunnett test was performed to test for differences in biofilm biomass and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in . ( B, D ) Representative scanning electron microscopy (SEM) images of LAC ∆spa∆sbi ( B ) and Wood46 ( D ) biofilms established on glass coverslips following 24 hr incubation at 37°C. SA, S. aureus ; EPS, extracellular polymeric substance structure.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A, C ) Biofilm of S. aureus strain LAC ∆spa∆sbi ( A ) and Wood46 ( C ) was grown for 24 hr with buffer or DNase (1 mg/mL). DspB (30 nM) was added after 24 hr of biofilm formation. Adherent biofilm biomass was measured by crystal violet staining. Data represent mean + SD of three independent experiments. Triplicates were averaged and expressed as relative biomass by dividing the OD595 of treated samples by the OD595 of control samples. One-way ANOVA followed by Dunnett test was performed to test for differences in biofilm biomass and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in . ( B, D ) Representative scanning electron microscopy (SEM) images of LAC ∆spa∆sbi ( B ) and Wood46 ( D ) biofilms established on glass coverslips following 24 hr incubation at 37°C. SA, S. aureus ; EPS, extracellular polymeric substance structure.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Staining, Control, Electron Microscopy, Incubation

( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments. ( C, D ) Biofilm was grown for 24 hr and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green), and mAb binding was detected by staining with Alexa Fluor 647-conjugated goat-anti-human-kappa F(ab′) 2 antibody (red). Orthogonal views are representative for a total of three Z-stacks per condition and at least two independent experiments. Scale bars: 10 μm.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments. ( C, D ) Biofilm was grown for 24 hr and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green), and mAb binding was detected by staining with Alexa Fluor 647-conjugated goat-anti-human-kappa F(ab′) 2 antibody (red). Orthogonal views are representative for a total of three Z-stacks per condition and at least two independent experiments. Scale bars: 10 μm.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence, Staining

( A ) Planktonic bacteria of LAC ∆spa∆sbi (left) and Wood46 (right) were grown to exponential phase and incubated with a concentration range of F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilm of Wood46 and LAC ∆spa∆sbi were grown for 24 h and incubated with a concentration range of F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Planktonic bacteria of LAC ∆spa∆sbi (left) and Wood46 (right) were grown to exponential phase and incubated with a concentration range of F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilm of Wood46 and LAC ∆spa∆sbi were grown for 24 h and incubated with a concentration range of F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence

( A ) Wood46 biofilm was grown for 24 h and incubated with control IgG1, IgG3 and anti-SpA IgG3. Mab binding was detected using anti-human-kappa-AF647 antibodies and a plate reader. ( B ) Planktonic exponential Wood46 bacteria were incubated with control IgG1, IgG3 and anti-SpA IgG3. Mab binding was detected using anti-human-kappa-AF647 antibodies and flow cytometry. Data represent mean + SD of three independent experiments.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Wood46 biofilm was grown for 24 h and incubated with control IgG1, IgG3 and anti-SpA IgG3. Mab binding was detected using anti-human-kappa-AF647 antibodies and a plate reader. ( B ) Planktonic exponential Wood46 bacteria were incubated with control IgG1, IgG3 and anti-SpA IgG3. Mab binding was detected using anti-human-kappa-AF647 antibodies and flow cytometry. Data represent mean + SD of three independent experiments.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Incubation, Control, Binding Assay, Bacteria, Flow Cytometry

( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of CR5132-IgG1. Monoclonal antibody (mAb) binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of CR5132-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least three independent experiments. ( C, D ) Biofilm was grown for 24hr and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green), and mAb binding was detected by staining with Alexa Fluor 647-conjugated goat-anti-human-kappa F(ab′) 2 antibody (red). Orthogonal views are representative for a total of three Z-stacks per condition and at least two independent experiments. Scale bars: 10 μm.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of CR5132-IgG1. Monoclonal antibody (mAb) binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of CR5132-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least three independent experiments. ( C, D ) Biofilm was grown for 24hr and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green), and mAb binding was detected by staining with Alexa Fluor 647-conjugated goat-anti-human-kappa F(ab′) 2 antibody (red). Orthogonal views are representative for a total of three Z-stacks per condition and at least two independent experiments. Scale bars: 10 μm.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence, Staining

( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: ( A ) Planktonic bacteria of Wood46 (left) and LAC ∆spa∆sbi (right) were grown to exponential phase and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. ( B ) Biofilms of Wood46 (left) and LAC ∆spa∆sbi (right) were grown for 24 hr and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of three independent experiments.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence

Planktonic bacteria of Wood46 ( A ) LAC ∆spa∆sbi ( B ) were grown to stationary phase and incubated with a concentration range of mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. Data represent mean + SD of three independent experiments.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: Planktonic bacteria of Wood46 ( A ) LAC ∆spa∆sbi ( B ) were grown to stationary phase and incubated with a concentration range of mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. Data represent mean + SD of three independent experiments.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry

Biofilms of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were grown for 24 hr and incubated with a concentration range of IgG1 mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. Data are expressed as area under the curve (AUC) of the binding curve (mean + SD) of three independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: Biofilms of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were grown for 24 hr and incubated with a concentration range of IgG1 mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. Data are expressed as area under the curve (AUC) of the binding curve (mean + SD) of three independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Incubation, Concentration Assay, Binding Assay, Labeling, Control

Planktonic bacteria of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were grown to exponential phase and incubated with a concentration range of IgG1 mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry. Data are expressed as area under the curve (AUC) of the binding curve (mean + SD) of three independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: Planktonic bacteria of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were grown to exponential phase and incubated with a concentration range of IgG1 mAbs. mAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry. Data are expressed as area under the curve (AUC) of the binding curve (mean + SD) of three independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *p≤0.05, **p≤0.01, ***p≤0.001, or ****p≤0.0001. Exact p-values are displayed in .

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Control

Monoclonal antibody (mAb) binding to biofilm and planktonic bacteria. Significant binding (p <0.05) of IgG1 mAbs compared to control IgG1 s indicated with ‘+,’ weak binding (p <0.05, p <0.99) is indicated with ‘+/-,’ and no significant binding (p> 0.99) is indicated with ‘–.’.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: Monoclonal antibody (mAb) binding to biofilm and planktonic bacteria. Significant binding (p <0.05) of IgG1 mAbs compared to control IgG1 s indicated with ‘+,’ weak binding (p <0.05, p <0.99) is indicated with ‘+/-,’ and no significant binding (p> 0.99) is indicated with ‘–.’.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Binding Assay, Bacteria, Control

The total AF647 signal of obtained Z-stack profiles of biofilms Wood46 ( A ) and LAC ∆spa∆sbi ( B ) was calculated using Leica LAS AF imaging software. Data are representative for two independent experiments.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: The total AF647 signal of obtained Z-stack profiles of biofilms Wood46 ( A ) and LAC ∆spa∆sbi ( B ) was calculated using Leica LAS AF imaging software. Data are representative for two independent experiments.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Imaging, Software

Biofilm cultures of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were incubated with 10 µg/mL AF647-conjugated IgG1 monoclonal antibodies (mAbs) in buffer or buffer containing 250 µg/mL pooled IgG. Data are expressed as % relative to mAb binding in buffer of three independent experiments performed in duplicate.

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet: Biofilm cultures of Wood46 ( A ) and LAC ∆spa∆sbi ( B ) were incubated with 10 µg/mL AF647-conjugated IgG1 monoclonal antibodies (mAbs) in buffer or buffer containing 250 µg/mL pooled IgG. Data are expressed as % relative to mAb binding in buffer of three independent experiments performed in duplicate.

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Incubation, Bioprocessing, Binding Assay

Journal: eLife

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

doi: 10.7554/eLife.67301

Figure Lengend Snippet:

Article Snippet: S. aureus strains Wood46 (ATCC 10832) ( ; ; ), USA300 LAC (AH1263) , and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study.

Techniques: Recombinant, Software, Staining

Production of mAbs and validation of biofilm. (A) Human IgG1 antibodies are large (150 kDa) proteins, consisting of two functional domains. The fragment antigen binding (Fab) region confers antigen specificity, while the crystallizable fragment (Fc) region drives interactions with the immune system. Each IgG1 is composed of 2 identical heavy chains and two identical light chains, which all consist of a constant (CH, CL) and a variable (VH, VL) domain. A panel of 6 human IgG1 mAbs that recognize polysaccharide and protein components on the cell surface of S. aureus and two non-specific isotype controls was produced. Variable heavy (VH) and light (VL) chain sequences obtained from different scientific and patent publications were cloned in homemade expression vectors containing human heavy chain (HC) and light chain (LC) constant regions, respectively. (B,C) Biofilm of Wood46 (B) and LACΔspaΔsbi (C) were grown for 24h and incubated with 66 nM F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments. A ratio paired t-test was performed to test for differences in antibody binding versus control and displayed only when significant as *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, or ****P ≤ 0.0001.

Journal: bioRxiv

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

doi: 10.1101/2021.02.09.429966

Figure Lengend Snippet: Production of mAbs and validation of biofilm. (A) Human IgG1 antibodies are large (150 kDa) proteins, consisting of two functional domains. The fragment antigen binding (Fab) region confers antigen specificity, while the crystallizable fragment (Fc) region drives interactions with the immune system. Each IgG1 is composed of 2 identical heavy chains and two identical light chains, which all consist of a constant (CH, CL) and a variable (VH, VL) domain. A panel of 6 human IgG1 mAbs that recognize polysaccharide and protein components on the cell surface of S. aureus and two non-specific isotype controls was produced. Variable heavy (VH) and light (VL) chain sequences obtained from different scientific and patent publications were cloned in homemade expression vectors containing human heavy chain (HC) and light chain (LC) constant regions, respectively. (B,C) Biofilm of Wood46 (B) and LACΔspaΔsbi (C) were grown for 24h and incubated with 66 nM F598-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments. A ratio paired t-test was performed to test for differences in antibody binding versus control and displayed only when significant as *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, or ****P ≤ 0.0001.

Article Snippet: This screen revealed strain Wood46 (ATCC 10832) as a robust biofilm former on uncoated, polystyrene microtiter plates (Fig. S1a) .

Techniques: Biomarker Discovery, Functional Assay, Binding Assay, Produced, Clone Assay, Expressing, Incubation, Labeling, Fluorescence, Control

Journal: bioRxiv

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

doi: 10.1101/2021.02.09.429966

Figure Lengend Snippet: IgG1 mAbs against WTA bind S. aureus in planktonic and biofilm mode. (A) Planktonic bacteria of Wood46 (left) and LACΔ spa Δ sbi (right) were grown to exponential phase and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. (B) Biofilm of Wood46 (left) and LACΔ spa Δ sbi (right) were grown for 24 h and incubated with a concentration range of 4461-IgG1 or 4497-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments. (C,D,E,F) Biofilm was grown for 24 h and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green) and mAb binding was detected by staining with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (red). (C,E) Orthogonal views are representative for a total of three Z-stacks per condition and at least 2 independent experiments. Scale bars: 10 μm. (D,F) Z-stack profile plotting the total fluorescence of Syto9 (green, dotted line) and AF647 (red line) per frame versus the depth (μm) of the corresponding Z-stack. Vertical green and red lines represent the center of mass of the total fluorescent signal.

Article Snippet: This screen revealed strain Wood46 (ATCC 10832) as a robust biofilm former on uncoated, polystyrene microtiter plates (Fig. S1a) .

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence, Staining

Journal: bioRxiv

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

doi: 10.1101/2021.02.09.429966

Figure Lengend Snippet: CR5132-IgG1 discriminates between planktonic bacteria and biofilm. (A) Planktonic bacteria of Wood46 (left) and LACΔ spa Δ sbi (right) were grown to exponential phase and incubated with a concentration range of CR5132-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. (B) Biofilm of Wood46 (left) and LACΔ spa Δ sbi (right) were grown for 24 h and incubated with a concentration range of CR5132-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments. (C,D,E,F) Biofilm was grown for 24 h and incubated with 66 nM IgG1 mAb. Bacteria were visualized by Syto9 (green) and mAb binding was detected by staining with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (red). (C,E) Orthogonal views are representative for a total of three Z-stacks per condition and at least 2 independent experiments. Scale bars: 10 μm. (D,F) Z-stack profile plotting the total fluorescence of Syto9 (green, dotted line) and AF647 (red line) per frame versus the depth (μm) of the corresponding Z-stack. Vertical green and red lines represent the center of mass of the total fluorescent signal.

Article Snippet: This screen revealed strain Wood46 (ATCC 10832) as a robust biofilm former on uncoated, polystyrene microtiter plates (Fig. S1a) .

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence, Staining

IgG1 mAbs against protein components bind planktonic bacteria as well as biofilm. (A) Planktonic bacteria of Wood46 (left) and LACΔ spa Δ sbi (right) were grown to exponential phase and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. (B) Biofilm of Wood46 (left) and LACΔ spa Δ sbi (right) were grown for 24 h and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments.

Journal: bioRxiv

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

doi: 10.1101/2021.02.09.429966

Figure Lengend Snippet: IgG1 mAbs against protein components bind planktonic bacteria as well as biofilm. (A) Planktonic bacteria of Wood46 (left) and LACΔ spa Δ sbi (right) were grown to exponential phase and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and flow cytometry and plotted as geoMFI of the bacterial population. (B) Biofilm of Wood46 (left) and LACΔ spa Δ sbi (right) were grown for 24 h and incubated with a concentration range of rF1-IgG1 or T1-2-IgG1. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader and plotted as fluorescence intensity per well. Data represent mean + SD of at least 3 independent experiments.

Article Snippet: This screen revealed strain Wood46 (ATCC 10832) as a robust biofilm former on uncoated, polystyrene microtiter plates (Fig. S1a) .

Techniques: Bacteria, Incubation, Concentration Assay, Binding Assay, Labeling, Flow Cytometry, Fluorescence

Comparative binding of IgG1 mAbs to S. aureus biofilm. Biofilm of Wood46 (A) and LACΔ spa Δ sbi (B) were grown for 24 h and incubated with a concentration range of IgG1 mAbs. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. Data (A) were expressed as AUC of the binding curve (mean + SD) of at least 3 independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, or ****P ≤ 0.0001.

Journal: bioRxiv

Article Title: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

doi: 10.1101/2021.02.09.429966

Figure Lengend Snippet: Comparative binding of IgG1 mAbs to S. aureus biofilm. Biofilm of Wood46 (A) and LACΔ spa Δ sbi (B) were grown for 24 h and incubated with a concentration range of IgG1 mAbs. MAb binding was detected using APC-labeled anti-human IgG antibodies and a plate reader. Data (A) were expressed as AUC of the binding curve (mean + SD) of at least 3 independent experiments. One-way ANOVA followed by Dunnett test was performed to test for differences in antibody binding versus control and displayed only when significant as *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, or ****P ≤ 0.0001.

Article Snippet: This screen revealed strain Wood46 (ATCC 10832) as a robust biofilm former on uncoated, polystyrene microtiter plates (Fig. S1a) .

Techniques: Binding Assay, Incubation, Concentration Assay, Labeling, Control

a Biofilms for the ATCC 10832 (non-MRSA) strain was allowed to form on glass-bottomed chambers for 24 h prior to be treated with the indicated antimicrobials or their combinations (right set of panels) or the respective control-vehicles (left set of panels). The biofilms were subsequently stained using the LIVE/DEAD biofilm staining kit and confocal microscope images were taken using a 63× oil immersion objective. b Same as in a with the exception that the strain used was the MRSA strain ATCC 33591. Scale bars correspond to 10 μm. The control vehicles were the same as described in Figs. and .

Journal: NPJ Biofilms and Microbiomes

Article Title: A bacteriocin-based antimicrobial formulation to effectively disrupt the cell viability of methicillin-resistant Staphylococcus aureus (MRSA) biofilms

doi: 10.1038/s41522-020-00166-4

Figure Lengend Snippet: a Biofilms for the ATCC 10832 (non-MRSA) strain was allowed to form on glass-bottomed chambers for 24 h prior to be treated with the indicated antimicrobials or their combinations (right set of panels) or the respective control-vehicles (left set of panels). The biofilms were subsequently stained using the LIVE/DEAD biofilm staining kit and confocal microscope images were taken using a 63× oil immersion objective. b Same as in a with the exception that the strain used was the MRSA strain ATCC 33591. Scale bars correspond to 10 μm. The control vehicles were the same as described in Figs. and .

Article Snippet: Fig. 3 Confocal laser scanning microscope images of S. aureus biofilms exposed to antimicrobial treatments. a Biofilms for the ATCC 10832 (non-MRSA) strain was allowed to form on glass-bottomed chambers for 24 h prior to be treated with the indicated antimicrobials or their combinations (right set of panels) or the respective control-vehicles (left set of panels).

Techniques: Staining, Microscopy

Review

atcc 10832 strain (ATCC)

Structured Review

Images

Related Articles

Cell Culture:

Similar Products

Image Search Results